Darman Faraz Kave

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Urinary Oxalate

Method:Manual/Automated

Principle:
Urine oxalate is converted to carbon dioxide and hydrogen peroxide . Hydrogen peroxide in the presence of peroxidase oxidizes a chromogen to form a colored complex. The intensity of the color is directly proportional to the concentration of oxalate and is determined calorimetrically at 590 nm.


Specimens:
Twenty four-hour urine sample collected in a bottles containing 10 ml concentrated HCl can be used.

Contents:
Reagent 1:  Buffer Solution                           25 ml
Reagent 2:  Lyophilized Enzyme                    5 Vials
Reagent 3:  Active Charcoal                          12 g
Reagent 4:  Oxalate Standard(0.25 mM)        5 ml
Reagent 5:  Sample Diluent                          60 ml
Number of Assays:    Manual                        20
Automated                                                  60
Catalogue Number:                                     DFK-103