Darman Faraz Kave

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Triglycerides

Method:Enzymatic, Colorimetric, End-point, Manual/Automated

Principle:
Triglycerides are hydrolysed to glycerol and free fatty acids by the action of lipase. The liberated glycerol is then phosphorylated by adenosine triphosphate (ATP) in the presence of glycerol kinase to form glycerol-3-phosphate (G-3-P) and adenine diphposphate. G-3-P is then oxidized by glycerol-3-phosphate oxidase to form dihydroxy acetone phosphate (DHAP) and hydrogen peroxide (H2O2). H2O2 is then mixed with a chromogen (4-aminophenazone) in the presence of p-chlorophenol to form a colored complex. The intensity of the color is measured colorimetrically at 505nm and is directly proportional to the concentration of triglycerides.

Specimens:
Serum or plasma may be used. Hemolyzed samples should not be used.

Contents:
Reagent 1: Enzyme solution                         3 x 100ml
Reagent 2: Standard (200 mg/dl)                 1 x 10ml
Number of Assays: Manual                          300
Automated Dependent on Instrument Type
Catalogue Number:                                     DFK-124